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Mg2+/Mn2+依赖性蛋白磷酸酶1B(PPM1B)检测试剂盒(酶联免疫吸附试验法)
货 号:
SEH327Hu-96T
规 格:
96T
品 牌:
cloud-clone
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英文名称: | ELISA Kit for Protein Phosphatase, Mg2+/Mn2+ Dependent 1B (PPM1B) |
基因别称: | PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX; Protein Phosphatase 2C,Beta Isoform; Protein Phosphatase 1B(formerly 2C),Magnesium-Dependent,Beta Isoform |
实验步骤: | 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC; 8. Add 50µL Stop Solution. Read at 450nm immediately. |
检测范围: | 0.156-10ng/mL |
最低检测限: | The minimum detectable dose of this kit is typically less than 0.055ng/mL |
适应生物: | Homo sapiens (Human) |
样本: | tissue homogenates, cell lysates and other biological fluids |
实验时长: | 3h |
种类: | 参考价格:2940/48T ; 4200/96T ; 18900/96T*5 ; 35700/96T*10 ; 294000/96T*100 ; |
质量控制: | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
产品应用: | ELISA , 酶联免疫 |
现货: | 5-7个工作日 |
运输条件: | 蓝冰运输 |
保存条件: | 4度和-20度分开保存,避免反复冻融 |
有效期: | 6个月 |
限制: | 仅供科研使用 |
标本量: | 详见说明书 |
Research_Areas: | Enzyme & Kinase; |
Emit: | Protein Phosphatase, Mg2+/Mn2+ Dependent 1B |