产品展示首页 > USCN & cloud-clone 试剂盒 & 抗体 & 蛋白 > cloud-clone 试剂盒、抗体、蛋白 > Vacuolar Protein Sorting 13D (VPS13D)
液泡蛋白分拣蛋白13D(VPS13D)检测试剂盒(酶联免疫吸附试验法)
货 号：
SEN411Mu-96T
规 格：
96T
品 牌：
cloud-clone
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| 英文名称： | ELISA Kit for Vacuolar Protein Sorting 13D (VPS13D) |
| 实验步骤： | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37oC; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC; 7. Add 50µL Stop Solution. Read at 450 nm immediately. |
| 检测范围： | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
| 最低检测限： | The minimum detectable dose of this kit is typically less than 0.057ng/mL |
| 适应生物： | Mus musculus (Mouse ，小鼠) |
| 样本： | Tissue homogenates, cell lysates and other biological fluids. |
| 实验时长： | 3hours |
| 种类： | 参考价格：3326.4/48T ; 4752/96T ; 21384/96T*5 ; 40392/96T*10 ; 332640/96T*100 ; |
| 特异性： | This assay has high sensitivity and excellent specificity for detection of Vacuolar Protein Sorting 13D (VPS13D). No significant cross-reactivity or interference between Vacuolar Protein Sorting 13D (VPS13D) and analogues was observed. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vacuolar Protein Sorting 13D (VPS13D) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vacuolar Protein Sorting 13D (VPS13D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 稳定性： | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 实验原理： | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin (INS) in the sample. |
| 质量控制： | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
| 产品应用： | ELISA , 酶联免疫 |
| 现货： | 5-7个工作日 |
| 运输条件： | 蓝冰运输 |
| 保存条件： | 4度和-20度分开保存，避免反复冻融 |
| 有效期： | 6个月 |
| 限制： | 仅供科研使用 |
| 标本量： | 详见说明书 |
| 需自备的设备及试剂： | 1、450±10nm滤光片的酶标仪(建议仪器使用前提前预热); 2、单道或多道微量加液器及吸头; 3、稀释样品的EP管; 4、蒸馏水或去离子水; 5、吸水纸; 6、盛放洗液的容器 |
| Research_Areas： | Signal transduction; |
