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| 英文名称： | Mini Samples ELISA Kit for Adiponectin (ADPN) |
| 基因别称： | GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1 |
| 实验步骤： | 1. Prepare all reagents, samples and standards; 2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C; 3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 20µL Stop Solution. Read at 450nm immediately. |
| 检测范围： | 0.156-10ng/mL |
| 最低检测限： | The minimum detectable dose of this kit is typically less than 0.059ng/mL |
| 适应生物： | Mus musculus (Mouse) |
| 样本： | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 种类： | Double-antibody Sandwich |
| 特异性： |
This assay has high sensitivity and excellent specificity for detection of Mini Samples Adiponectin (ADPN). |
| 精度： |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Adiponectin (ADPN) were tested 20 times on one plate, respectively. |
| 稳定性： |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
| 实验原理： | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Adiponectin (ADPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Adiponectin (ADPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Adiponectin (ADPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Adiponectin (ADPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| 产品应用： | Enzyme-linked immunosorbent assay for Antigen Detection. |
| 应用备注： | 48T, 96T, 96T×5, 96T×10, 96T×100 |
| 期货： | 5-7天 |
| 运输条件： | 蓝冰运输 |
| 保存条件： | 按要求4度和-20度分开保存 |
| 有效期： | 12个月 |
| 限制： | 仅用科研 ( ONLY USE R&D research ) |
| 需自备的设备及试剂： | 450nm酶标仪 & 移液器等 |
| Research_Areas： | Metabolic pathway;Infection immunity;Cardiovascular biology; |
| Emit： | Adiponectin |
| Excite： | Q60994 |
