干扰素α(IFNa)检测试剂盒(酶联免疫吸附试验法,快捷型) - 鲎试剂，ELISA试剂盒,ELISA试剂盒价格,国产ELISA试剂盒,进口ELISA试剂盒

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干扰素α(IFNa)检测试剂盒(酶联免疫吸附试验法,快捷型)

货号：

USCN-IEA033Ga

规格：

96wells

品牌：

Uscn

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英文名称：

Instant ELISA Kit for Interferon Alpha (IFNa)

基因别称：

IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D

实验步骤：

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.

检测范围：

15.6-1,000pg/mL

最低检测限：

The minimum detectable dose of this kit is typically less than 5.4pg/mL

适应生物：

Chicken (Gallus)

样本：

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

实验时长：

1h, 10min

种类：

Double-antibody Sandwich

特异性：

This assay has high sensitivity and excellent specificity for detection of Instant Interferon Alpha (IFNa).
No significant cross-reactivity or interference between Instant Interferon Alpha (IFNa) and analogues was observed.

精度：

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

稳定性：

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

实验原理：

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Interferon Alpha (IFNa). Standards or samples and HRP-labeled detection antibody specific to Instant Interferon Alpha (IFNa) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Interferon Alpha (IFNa), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Interferon Alpha (IFNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

产品应用：

Enzyme-linked immunosorbent assay for Antigen Detection.

应用备注：

48T, 96T, 96T×5, 96T×10, 96T×100

期货：

5-7天

运输条件：

蓝冰运输

保存条件：

按要求4度和-20度分开保存

有效期：

12个月

限制：

仅用科研 ( ONLY USE R&D research )

需自备的设备及试剂：

450nm酶标仪 & 移液器等

Research_Areas：

Cytokine;Infection immunity;

Emit：

Interferon Alpha

Excite：

P42165

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