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| 英文名称： | ELISA Kit for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) |
| 实验步骤： | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately. |
| 检测范围： | 246.9-20,000pg/mL |
| 最低检测限： | The minimum detectable dose of this kit is typically less than 108.1pg/mL |
| 适应生物： | Rattus norvegicus (Rat) |
| 样本： | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 实验时长： | 2h |
| 种类： | Competitive Inhibition |
| 特异性： |
This assay has high sensitivity and excellent specificity for detection of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c). |
| 精度： |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) were tested 20 times on one plate, respectively. |
| 稳定性： |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
| 实验原理： | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and unlabeled Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) (Standards or samples) with the pre-coated antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) in the sample. |
| 产品应用： | Enzyme-linked immunosorbent assay for Antigen Detection. |
| 应用备注： | 48T, 96T, 96T×5, 96T×10, 96T×100 |
| 期货： | 5-7天 |
| 运输条件： | 蓝冰运输 |
| 保存条件： | 按要求4度和-20度分开保存 |
| 有效期： | 12个月 |
| 限制： | 仅用科研 ( ONLY USE R&D research ) |
| Comment： | 需要代测请联系说明 |
| 需自备的设备及试剂： | 450nm酶标仪 & 移液器等 |
| Research_Areas： | Developmental science; |
| Emit： | Mitochondrial Open Reading Frame Of The 12S rRNA-c |
| Excite： | - |
