磷肌酸(PCr)检测试剂盒(酶联免疫吸附试验法) - 鲎试剂，ELISA试剂盒,ELISA试剂盒价格,国产ELISA试剂盒,进口ELISA试剂盒

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磷肌酸(PCr)检测试剂盒(酶联免疫吸附试验法)

货号：

USCN-CEV808Ge

规格：

96wells

品牌：

Uscn

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英文名称：

ELISA Kit for Phosphocreatine (PCr)

基因别称：

CP; Creatine Phosphate; Phosphorylcreatine; Creatine-P; Phosphagen; Fosfocreatine

实验步骤：

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

检测范围：

123.5-10,000ng/mL

最低检测限：

The minimum detectable dose of this kit is typically less than 50.3ng/mL

适应生物：

Pan-species (General)

样本：

serum, plasma and other biological fluids

实验时长：

种类：

Competitive Inhibition

特异性：

This assay has high sensitivity and excellent specificity for detection of Phosphocreatine (PCr).
No significant cross-reactivity or interference between Phosphocreatine (PCr) and analogues was observed.

精度：

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphocreatine (PCr) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphocreatine (PCr) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

稳定性：

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

实验原理：

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Phosphocreatine (PCr) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Phosphocreatine (PCr) and unlabeled Phosphocreatine (PCr) (Standards or samples) with the pre-coated antibody specific to Phosphocreatine (PCr). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Phosphocreatine (PCr) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Phosphocreatine (PCr) in the sample.

产品应用：

Enzyme-linked immunosorbent assay for Antigen Detection.

应用备注：

48T, 96T, 96T×5, 96T×10, 96T×100

期货：

5-7天

运输条件：

蓝冰运输

保存条件：

按要求4度和-20度分开保存

有效期：

12个月

限制：

仅用科研 ( ONLY USE R&D research )

Comment：

需要代测请联系说明

需自备的设备及试剂：

450nm酶标仪 & 移液器等

Emit：

Phosphocreatine

Excite：

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USCN & cloud-clone 试剂盒 & 抗体 & 蛋白

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Charles River Endosafe(美国)

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内毒素检测仪器

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