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| 英文名称： | ELISA Kit for Luteinizing Hormone Beta Polypeptide (LHb) |
| 基因别称： | LH-B; CGB4; LSH-B; hLHB; Lutropin Subunit Beta; Interstitial Cell Stimulating Hormone,Beta Chain |
| 实验步骤： | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately. |
| 检测范围： | 246.91-20000pg/mL |
| 最低检测限： | The minimum detectable dose of this kit is typically less than 90.4pg/mL |
| 适应生物： | Homo sapiens (Human) |
| 样本： | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 实验时长： | 2h |
| 种类： | Competitive Inhibition |
| 特异性： |
This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone Beta Polypeptide (LHb). |
| 精度： |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone Beta Polypeptide (LHb) were tested 20 times on one plate, respectively. |
| 稳定性： |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
| 实验原理： | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone Beta Polypeptide (LHb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone Beta Polypeptide (LHb) and unlabeled Luteinizing Hormone Beta Polypeptide (LHb) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone Beta Polypeptide (LHb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone Beta Polypeptide (LHb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone Beta Polypeptide (LHb) in the sample. |
| 产品应用： | Enzyme-linked immunosorbent assay for Antigen Detection. |
| 应用备注： | 48T, 96T, 96T×5, 96T×10, 96T×100 |
| 期货： | 5-7天 |
| 运输条件： | 蓝冰运输 |
| 保存条件： | 按要求4度和-20度分开保存 |
| 有效期： | 12个月 |
| 限制： | 仅用科研 ( ONLY USE R&D research ) |
| Comment： | 需要代测请联系说明 |
| 需自备的设备及试剂： | 450nm酶标仪 & 移液器等 |
| Research_Areas： | Reproductive science; |
| Emit： | Luteinizing Hormone Beta Polypeptide |
| Excite： | P01229 |
