α白蛋白(AFM)检测试剂盒(酶联免疫吸附试验法) - 鲎试剂，ELISA试剂盒,ELISA试剂盒价格,国产ELISA试剂盒,进口ELISA试剂盒

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α白蛋白(AFM)检测试剂盒(酶联免疫吸附试验法)

货号：

USCN-CEC284Ra

规格：

96wells

品牌：

Uscn

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英文名称：

ELISA Kit for Afamin (AFM)

基因别称：

ALB2; ALBA; ALF; A-ALB; Alpha-Albumin

实验步骤：

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 5 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450 nm immediately.

检测范围：

2.47-200ng/mL

最低检测限：

The minimum detectable dose of this kit is typically less than 0.93ng/mL

适应生物：

Rattus norvegicus (Rat)

样本：

Serum, plasma and other biological fluids.

实验时长：

种类：

Competitive Inhibition

特异性：

This assay has high sensitivity and excellent specificity for detection of Afamin (AFM).
No significant cross-reactivity or interference between Afamin (AFM) and analogues was observed.

精度：

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Afamin (AFM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Afamin (AFM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

稳定性：

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

实验原理：

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Afamin (AFM) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Afamin (AFM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

产品应用：

Enzyme-linked immunosorbent assay for Antigen Detection.

应用备注：

48T, 96T, 96T×5, 96T×10, 96T×100

期货：

5-7天

运输条件：

蓝冰运输

保存条件：

按要求4度和-20度分开保存

有效期：

12个月

限制：

仅用科研 ( ONLY USE R&D research )

Comment：

需要代测请联系说明

需自备的设备及试剂：

450nm酶标仪 & 移液器等

Research_Areas：

Tumor immunity;Infection immunity;Hepatology;

Emit：

Afamin

Excite：

P36953

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USCN & cloud-clone 试剂盒 & 抗体 & 蛋白

细菌内毒素检测试剂（安度斯）

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内毒素检测专用耗材

内毒素检测仪器

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