R-脊椎蛋白1(RSPO1)检测试剂盒(酶联免疫吸附试验法) - 鲎试剂，ELISA试剂盒,ELISA试剂盒价格,国产ELISA试剂盒,进口ELISA试剂盒

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R-脊椎蛋白1(RSPO1)检测试剂盒(酶联免疫吸附试验法)

货号：

USCN-SEM171Mu

规格：

96wells

品牌：

Uscn

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英文名称：

ELISA Kit for R-Spondin 1 (RSPO1)

基因别称：

RSPONDIN; Roof plate-specific spondin-1

实验步骤：

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

检测范围：

0.156-10ng/mL

最低检测限：

The minimum detectable dose of this kit is typically less than 0.054ng/mL

适应生物：

Mus musculus (Mouse)

样本：

tissue homogenates, cell lysates, cell culture supernates and other biological fluids

实验时长：

种类：

Double-antibody Sandwich

特异性：

This assay has high sensitivity and excellent specificity for detection of R-Spondin 1 (RSPO1).
No significant cross-reactivity or interference between R-Spondin 1 (RSPO1) and analogues was observed.

精度：

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level R-Spondin 1 (RSPO1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level R-Spondin 1 (RSPO1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

稳定性：

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

实验原理：

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 1 (RSPO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 1 (RSPO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 1 (RSPO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of R-Spondin 1 (RSPO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

产品应用：

Enzyme-linked immunosorbent assay for Antigen Detection.

应用备注：

48T, 96T, 96T×5, 96T×10, 96T×100

期货：

5-7天

运输条件：

蓝冰运输

保存条件：

按要求4度和-20度分开保存

有效期：

12个月

限制：

仅用科研 ( ONLY USE R&D research )

需自备的设备及试剂：

450nm酶标仪 & 移液器等

Research_Areas：

Signal transduction;

Emit：

R-Spondin 1

Excite：

Q9Z132

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