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| 英文名称： | ELISA Kit for Deleted In Malignant Brain Tumors 1 (DMBT1) |
| 基因别称： | GP340; SAG; Muclin; Glycoprotein 340; Hensin; Salivary agglutinin; Surfactant pulmonary-associated D-binding protein |
| 实验步骤： | 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately. |
| 检测范围： | 0.156-10ng/mL |
| 最低检测限： | The minimum detectable dose of this kit is typically less than 0.056ng/mL |
| 适应生物： | Mus musculus (Mouse) |
| 样本： | tissue homogenates, cell lysates and other biological fluids |
| 实验时长： | 3h |
| 种类： | Double-antibody Sandwich |
| 特异性： |
This assay has high sensitivity and excellent specificity for detection of Deleted In Malignant Brain Tumors 1 (DMBT1). |
| 精度： |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deleted In Malignant Brain Tumors 1 (DMBT1) were tested 20 times on one plate, respectively. |
| 稳定性： |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
| 实验原理： | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deleted In Malignant Brain Tumors 1 (DMBT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deleted In Malignant Brain Tumors 1 (DMBT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deleted In Malignant Brain Tumors 1 (DMBT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deleted In Malignant Brain Tumors 1 (DMBT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| 产品应用： | Enzyme-linked immunosorbent assay for Antigen Detection. |
| 应用备注： | 48T, 96T, 96T×5, 96T×10, 96T×100 |
| 期货： | 5-7天 |
| 运输条件： | 蓝冰运输 |
| 保存条件： | 按要求4度和-20度分开保存 |
| 有效期： | 12个月 |
| 限制： | 仅用科研 ( ONLY USE R&D research ) |
| 需自备的设备及试剂： | 450nm酶标仪 & 移液器等 |
| Research_Areas： | Tumor immunity; |
| Emit： | Deleted In Malignant Brain Tumors 1 |
| Excite： | - |
