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FFPE-FASP 蛋白质消化试剂盒

货号：

expedeon-44255

规格：

Kit

品牌：

expedeon(英国）

产品描述
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英文名称：

Expedeon FFPE-FASP™ Protein Digestion Kit

适应生物：

Protein Digestion

特异性：

Filter-Aided Sample Prep (FASP) is the enabling technology behind quantitative mass spectrometry analysis of archived tissues. The resulting filtrate is free of detergents, large molecules, and other substances that would interfere with mass spectrometry analysis of the proteome. Contents: Reagents and material to process 8 samples (8 pack)

实验原理：

FFPE Tissue Proteomics with FASP

Filter-Aided Sample Prep (FASP) is the enabling technology behind quantitative mass spectrometry analysis of archived tissues. Based on a spin-filter sample preparation method initially described by Manza, et al., and developed further and extended to FFPE tissue processing by Ostasiewicz, et al., this method features unbiased extraction, complete proteome solubilization, and highly efficient digestion. The resulting filtrate is free of detergents, large molecules, and other substances that would interfere with mass spectrometry analysis of the proteome.

Same-as-Fresh Quantification

FFPE tissue proteomes can now be quantified and analyzed by mass spectrometry. An extensive, quantitative mass spectrometry comparison study described by Ostasiewicz, et al. found no significant differences between the proteomes of fresh and FFPE tissue samples processed using FFPE-FASP. Proteins, peptides, and post-translational modifications were quantitatively preserved. Results were unaffected by hematoxylin staining, laser capture microdissection, or tissue storage time.

组成：

FASP PROTEIN DIGESTION KIT

Complete Protein Solubilization. Complete Trypsin Digestion. Every Time. Now you can use aggressive, whole proteome solubilization protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Based on the Universal Sample Preparation method developed by Wisniewski, Zoubman, Nagaraj and Mann, Protein Discovery's FASP Protein Digestion Kit enables efficient digestion of samples for proteome analysis, even in the presence of the most extreme contaminating species. This method is the perfect complement to lysis buffers containing strong detergents and reducing agents. No longer are you forced to choose between efficient extraction or digestion. The FASP kit allows you to do both at the highest efficiency.

FFPE-FASP Protein Digestion Kit

De-paraffinization and Uncrosslinking. Unbiased Extraction and Complete Solubilization. Same-As-Fresh Quantification. Filter-Aided Sample Prep (FASP) is the enabling technology behind quantitative mass spectrometry analysis of archived tissues. Based on a spin-filter sample preparation method initially described by Manza, et al., and developed further and extended to FFPE tissue processing by Ostasiewicz, et al., this method features unbiased extraction, complete proteome solubilization, and highly efficient digestion. The resulting filtrate is free of detergents, large molecules, and other substances that would interfere with mass spectrometry analysis of the proteome.

Additional Information

Shelf Life	1 Year from receipt
Storage	4°C

FASP PROTEIN DIGESTION KIT FFPE-FASP Protein Digestion Kit

产品应用：

MS的胰蛋白酶消化

现货：

请咨询

运输条件：

4°C

保存条件：

4°C

有效期：

1 Year from receipt

Publication

1.A Potential Role for the Interaction of Wolbachia Surface Proteins