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白介素35(IL35)检测试剂盒
货 号：
SEC008Hu-96T
规 格：
96T
品 牌：
cloud-clone & uscn
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| 英文名称： | ELISA Kit for Interleukin 35 (IL35) |
| 实验步骤： | 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC; 8. Add 50µL Stop Solution. Read at 450nm immediately. |
| 检测范围： | 15.6-1,000pg/mL |
| 最低检测限： | The minimum detectable dose of this kit is typically less than 5.8pg/mL |
| 适应生物： | Homo sapiens (Human , 人) |
| 样本： | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 实验时长： | 3hours |
| 种类： | 参考价格：2940/48T ; 4200/96T ; 18900/96T*5 ; 35700/96T*10 ; 294000/96T*100 ; |
| 特异性： | This assay has high sensitivity and excellent specificity for detection of Interleukin 35 (IL35). No significant cross-reactivity or interference between Interleukin 35 (IL35) and analogues was observed. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 35 (IL35) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 35 (IL35) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 稳定性： | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 实验原理： | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromogranin A (CHGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromogranin A (CHGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromogranin A (CHGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chromogranin A (CHGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| 质量控制： | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
| 产品应用： | ELISA , 酶联免疫 |
| 现货： | 5-7个工作日 |
| 运输条件： | 蓝冰运输 |
| 保存条件： | 4度和-20度分开保存，避免反复冻融 |
| 有效期： | 6个月 |
| 限制： | 仅供科研使用 |
| 标本量： | 详见说明书 |
| 需自备的设备及试剂： | 1、450±10nm滤光片的酶标仪(建议仪器使用前提前预热); 2、单道或多道微量加液器及吸头; 3、稀释样品的EP管; 4、蒸馏水或去离子水; 5、吸水纸; 6、盛放洗液的容器 |
| Emit： | Interleukin 35 |
